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. 2015 Aug 10;5:11754. doi: 10.1038/srep11754

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A. Representative crystal violet staining for DU145 and PC3 cells that migrated and attached to the bottom of transwell filters after 24 h of treatment. B. Statistical analysis of crystal violet staining intensity measured as the absorbance at 560 nm, (n = 3 for DU145 cells, n = 5 for PC3 cells). C. Wound-healing assays of cell motility in DU145 and PC3 cells, and representative images obtained at 0 and 24 or 36 h after scratching. The dashed line indicated the edge of the wound. D. Statistical analysis of the wound-healing rates of DU145 and PC3 cells, (n = 5 for DU145 cells, n = 3 for PC3 cells). E. Western blotting analysis of E-cadherin, claudin-1, Vimentin, Wnt-1, Snail, p-Smad2 and p-Smad3 in DU145 cells. F. Western blotting analysis of nuclear β-catenin in DU145 cells.