Figure 1. Schematic representation of peptide array-based intracellular functional peptide screening.

(A) Starting from peptide synthesis on cellulose membrane by peptide array synthesizer, this figure is an overview of how a peptide is spotted on the cellulose membrane. The word “Photo” means photo-cleavable linker. (B)(i) Peptides spotted on a membrane were cut by UV irradiation. (ii) Each peptide spot was punched out as a disk and placed in one well of a 96-well plate. (iii) These peptides were dissolved by adding the medium, and (iv) this solution was filtered. (v) Filtered peptide-containing solutions were added to a second 96-well plate containing cultured cells. Intracellular functions impacted by the peptides were analyzed.