Table 1.
Studies reporting miRNA expression in diseased vs. healthy gingival tissues
| Study | N (samples/subjects) | Method | Confirmation | Correction for multiple testing |
Findings | Comments |
|---|---|---|---|---|---|---|
| Stoecklin- Wasmer et al. (156) |
198 gingival tissue samples (158 ‘diseased’, 40 ‘healthy’) from 86 subjects |
Hybridization (Agilent microarrays) |
qPCR (probe-based) | Yes (FDR) | 159 differentially expressed miRNAs at FDR<0.05; 4 miRNAs were upregulated by >2- fold (hsa-miR-451, hsa-miR-223, hsa- miR-486-5p, hsa- miR-3917), and 7 miRNAs were downregulated by > 2-fold (hsa-miR- 1246, hsa-miR- 1260, hsa-miR-141, hsa-miR-1260b, hsa- miR-203, hsa-miR- 210, hsa-miR-205*) in disease vs. health |
Concurrent analysis of miRNA and mRNA expression; functional annotation by miRNA target Enrichment Analysis; gingival tissue samples obtained from untreated periodontitis |
| Perri et al. (131) |
40 gingival tissue samples (20 ‘diseased’, 20 ‘healthy’ gingival tissues) from 20 individuals (10 obese and 10 non-obese), with or without periodontitis |
qPCR array (SABiosciences); SYBR green- based assessment of 88 miRNAs |
No | No | Using miRNA expression at healthy gingiva from non-obese subjects as the reference, two miRNAs were upregulated in non- obese individuals with periodontitis (miR 30e, miR- 106b), nine in obese individuals with periodontitis (miR- 15a, miR-18a, miR- 22, miR-30d, miR- 30e, miR-103, miR- 106b, miR-130a, miR-142-3p, miR- 185, and miR-210), and two in obese, periodontally healthy individuals (miR-18a, miR-30e) |
|
| Xie et al. (191) | 20 gingival tissue samples samples (10 ‘diseased’, 10 ‘healthy’) from 20 subjects, half of whom had periodontitis; all samples from the same condition were pooled (two samples in total were analyzed) |
Hybridization (Exiqon microarray) |
qPCR (SYBR green based) |
No | 91 miRNAs upregulated and 34 down-regulated by at least twofold in disease vs. health |
No statistics possible due to pooling of the samples; gingival tissue samples obtained after completed initial therapy |
| Lee et al. (98) | No sample size reported | qPCR array (SABiosciences; 88 miRNAs, SYBR green- based) |
qPCR (probe-based) | No | 6 miRNAs differentially expressed > 8-fold, 22 miRNAs > 4-fold in disease vs. health |
No statistics reported |