Table 1. List of antibodies used.
Antigen | Clone/antibody | Host | Working concentration (μg/ml) | Supplier | ||
---|---|---|---|---|---|---|
IB | IF | IHC | ||||
Claudin 1 | JAY.8 | Rb | 0.25 | n/a | n/a | Life Technologies |
Claudin 3 | Z23.JM | Rb | 0.25 | 2.5 | n/a | Life Technologies |
Claudin 3 | Ab52231 | Rb | n/a | n/a | 1 | Abcam |
Claudin 4 | 3E2C1 | M | 0.5 | n/a | n/a | Life Technologies |
Claudin 5 | 4C3C2 | M | 0.5 | n/a | n/a | Life Technologies |
Claudin 7 | ZMD.241 | Rb | 0.25 | n/a | n/a | Life Technologies |
ZO-1 | ZO-1-1A12 | Rb | 1 | 1.25 | 10 | Life Technologies |
ZO-1α+ | Anti-ZO-1α+ | Rb | 1.3 | 2 | 0.04 | Hycult Biotech |
β-actin | AC-15 | M | 0.004 | n/a | n/a | Sigma Aldrich |
Primary antibodies used for immunoblotting (IB), immunofluorescence (IF) and immunohistochemistry (IHC). M, mouse; Rb, Rabbit