Table 3.
Antigen | Immunogen | Type; manufacturer; catalog No. | MW(kDa) | Dilution |
---|---|---|---|---|
PAX7 | Recombinant protein made in E.coli, containing the amino acids 352-523 of chicken PAX7 protein | Monoclonal mouse anti-PAX7; Developmental Studies Hybridoma Bank; catalog reference: Pax7-c | 55 | 1:500 |
GABA | GABA-BSA | Polyclonal rabbit anti-c-minobutyric acid. Sigma; catalog reference: A2052 | 0.0103 | 1:3000 |
Nkx2.2 | E.coli-derived recombinant chick NKX2.2 NK2 factor related | Monoclonal mouse anti-Nkx2.2; Developmental Studies Hybridoma Bank; catalog reference: 74.5A5 | 30 | 1:500 |
Islet1 | Amino acids 247-349 at the c-terminus of rat Islet1 | Monoclonal mouse-anti-Isl; Developmental Studies Hybridoma Bank; catalog reference: 40.2D6 | 39 | 1:500 |