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. Author manuscript; available in PMC: 2016 Jul 23.
Published in final edited form as: Neurosci Lett. 2015 Jun 9;600:104–109. doi: 10.1016/j.neulet.2015.06.013

Fig. 2. Inhibition of astrocyte scratch response to ECM substrates by β1 integrin blockade.

Fig. 2

(A) Quantification of 24 h wound recovery of mixed glial cultures were grow on cover-slips coated with Ln, TnC, Vn, or Fn and stimulated with a mechanical scratch with either with® integrin blocking antibody Ha2/5 or control IgM. Analysis of variance was used to determine whether Ha2/5 affected wound injury responses on the different ECM substrates. (B) Primary cultures were treated with Ha2/5 or Ha2/5 and IL-1β and 24 hour scratch wound recovery measured on different ECM substrates, as indicated. Following each treatment, wound recovery was measured following each treatment relative to initial wound diameter (at 0 hour) and comparisons made to controls for each treatment. (*P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001). (C) Representative photomicrographs of GFAP + astrocytes from each culture condition, as indicated, showing comparable gross morphology on each substrate with or without Ha2/5 treatment. Scale bar = 30 μm.