Fig. 1.

Histology of mice fed chow or high fat high calorie diet (HFHCD) for 16 and 52 weeks. (A,B) In chow-fed mice, the liver histology was normal at both 16 and 52 weeks, respectively. (C,D) In HFHCD-fed mice, the liver developed a light tan color and some nodularity by week 52. HFHCD-fed mice also developed macrovesicular steatosis, foci of small droplet steatosis, and scattered inflammation at 16 weeks (E) along with focal cytological ballooning (arrow) and pericellular fibrosis (E–F). By 52 weeks, there was prominence of macrovesicular and centrilobular small droplet steatosis along with extensive pericellular fibrosis and areas of bridging fibrosis (G–H).