Extended Data Figure 4.

(a) Gene ontology (GO) enrichment matrix for 150 binding experiments (75 factors) spanning 6,347 significant GO enrichments (BH-corrected, P < 0.05) across 713 GO terms (level ≥4). For each experiment, GO-term enrichment was performed on gene targets as defined by binding within 1 kb of TSSs (ChipPeakAnno) ³¹. Enrichments for biological process (BP) and molecular function (MF) ontologies are shown, with distinct sets of enrichments highlighted (i–viii). (b) GO term enrichments among targets of UNC-62 binding show dramatical changes in the functional role of UNC-62 regulatory activity through development. Biological process (BP) terms (levels ≥4) enriched in UNC-62 libraries are shown. The number of UNC-62 binding sites identified per stage is indicated in parenthesis. Although changes in targets between mid-larval and adult stages have been suggested previously²², our analyses (performed with uniformly called binding sites) and expanded data indicate that the most dramatic changes occur between embryo and L4 larval stages. (+) MEP-1 indicates experiments performed in strain OP102.