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. 2015 Aug 10;10(8):e0134878. doi: 10.1371/journal.pone.0134878

Fig 5. Rapid proteasome degradation of SMAD4 in DBA iPSCs.

Fig 5

DBA iPSCs, and the corrected DBA cells were cultured in the iPSC medium, and treated with proteasome inhibitors MG132 (4 μM) for 12h or PS341 (20 μM) for 4h. A-B) Increased level of SMAD4 in both DBA cells after treatment with proteasome inhibitor MG132 for 12h. C) Increased level of nuclear SMAD4 in DBA iPSCs with RPS19 mutation after PS341 treatment. D and E) Increase of nuclear and cytoplasmic TRIM33, and increase of nuclear USP9X in both DBA iPSCs. The PS341 treatment did not affect the levels of TRIM33 and USP9X.