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. 2015 Aug 10;10(8):e0134280. doi: 10.1371/journal.pone.0134280

Fig 4. Analysis of Nanos ortholog identified in C.hongkongensis transcriptome.

Fig 4

(A) Amino acid sequence alignment of the nanos RNA-binding domain predicted in the Ch-nanos with that of other Nanos orthologs. Two highly conserved CCHC zinc finger motifs are indicated by asterisks. For the GenBank accession numbers of the reference sequences, see S3 Table. (B) Domain structure of Ch-nanos. (C) Expression profile of in Ch-nanos adult tissues by qRT-PCR with mean±s.e.m. as error bars (n = 5). nd: not detected. (D) Histological analysis of C.hongkongensis female gonad at early developing stage. (E) Expression profile of Ch-nanos in early developing stage female gonad detected by ISH with antisense and sense probe. (F) Positive cells are stained in purple.OG: oogonia; EO: early vitellogenic oocyte.