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rHDL, rHDL/res or rHDL/res/NBD (10 g protein) was incubated with 10 μg of sLDLr, followed by co-IP with anti-c-Myc-Agarose. sLDLr-bound apoE was detected by Western blot using HRP-conjugated polyclonal apoE antibody (A). The corresponding blot using anti-c-Myc antibody is shown for comparison (B). The lane assignments are: Lane 1, rHDL; lane 2, rHDL/res; lane 3, rHDL/res/NBD.
