Fig 4. Expression of fusion constructs in C. trachomatis L2.

(A). Transcription of blaM fusions (grey bars) and mCherry (black bars) was measured by reverse transcription followed by quantitative real-time PCR. Levels were normalized to those of chlamydial 16s. Total RNA was isolated 24 hpi from HeLa cells infected with transformed C. trachomatis L2 at an MOI of 1. (B). Total protein was isolated 24 hpi from HeLa cells infected with transformed C. trachomatis L2 at an MOI of 1, and samples were probed with BlaM- or chlamydial Hsp60-specific monoclonal antibodies. Size standards are indicated in kDa.