Fig 5. Detection of BlaM fusions in the cytosol of the host cell.

24 hpi, HeLa cells infected with transformed C. trachomatis L2 were examined for the presence of cytosolic BlaM with the GeneBLAzer Detection Kit. Samples were treated with CCF2-AM for 30 minutes, fixed with 4% paraformaldehyde, and examined by confocal microscopy. Chlamydial expression of CT694-, CT695-, and TarP-BlaM constructs produced significant blue-fluorescent signal in the cytosol of infected cells. Bar = 10 μm.