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. Author manuscript; available in PMC: 2015 Aug 10.
Published in final edited form as: Acta Trop. 2014 Jul 19;138:78–82. doi: 10.1016/j.actatropica.2014.07.007

Fig. 1.

Fig. 1

Determination of optimal dried blood spot (DBS) dilutions (A) and storage conditions (B) for NIE DBS-ELISA. (A) Spiked pooled positive and negative control blood spots were prepared and eluted in PBS-T, and different dilutions tested in NIE antigen ELISA. (B) Control blood spots were stored under different conditions for 1, 3 and 7 days. At the conclusion of the storage period all dried blood spots were stored at 4 °C, with elutions and ELISAs of all spots performed in a single assay. Each experiment/storage condition/dilution was repeated twice, and ELISAs were done in triplicate. RT = room temperature. For reference the 0.21 cut-off line is indicated.