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Calibration curve and protein content
| Assays | Regression equations | r2 | Linearity ranges | Protein content (w/w)
|
||
|---|---|---|---|---|---|---|
| ACC | CCC | TCPC | ||||
| BCA | Y=0.0010X +0.0651 | 0.9993 | 62.50–2,000 μg/mL | 44.2% | 40.7% | 36.4% |
| Bradford | Y=0.0056X +0.0729 | 0.9995 | 20.00–100.0 μg/mL | 19.3% | 15.9% | 14.4% |
Abbreviations: ACC, Asini Corii Colla; BCA, bicinchoninic acid; CCC, Cervi Cornus Colla; TCPC, Testudinis Carapacis ET Plastri Colla; r2, correlation coefficient; w/w: weight/weight.
