Table 3.
List of potential biomarkers for individual GCMs
| Codes | Potential biomarkers | GCMs | NanoLC
|
Orbitrap MSD
|
PEAKS
|
|||
|---|---|---|---|---|---|---|---|---|
| tR (min) | Charges | Precursor MH2+ | Precursor mass | Mass error | ALC | |||
| A1 | GFPGAVGAKGEGGGAP | ACC | 18.07 | 2+ | 664.8349 | 1,327.652 | 2.4 ppm | 95% |
| A2 | FGSGLDKADGGAGAP | ACC | 26.13 | 2+ | 660.3151 | 1,318.615 | 0.3 ppm | 94% |
| A3 | LAGAPGFRAGP | ACC | 16.87 | 2+ | 523.2746 | 1,044.535 | −0.5 ppm | 94% |
| A4 | FSGLDGSVAGGAGAP | ACC | 19.72 | 2+ | 631.8045 | 1,261.594 | 0.6 ppm | 93% |
| A5 | QTGFPGAAGGVVGPP | ACC | 17.53 | 2+ | 680.3351 | 1,358.647 | 6.8 ppm | 93% |
| C1 | GGPGSVFGGPGADGVAGKP | CCC | 14.26 | 3+ | 539.2655 | 1,614.764 | 6.8 ppm | 94% |
| C2 | QPGTPGPEGLATQGP | CCC | 17.17 | 2+ | 719.8500 | 1,437.674 | 8.2 ppm | 92% |
| C3 | VAGEPAAADGLGPGGP | CCC | 15.41 | 2+ | 684.3293 | 1,366.637 | 5.5 ppm | 91% |
| C4 | FSGLDGASVGGAGAP | CCC | 22.90 | 2+ | 631.8044 | 1,261.594 | 0.5 ppm | 90% |
| T1 | QTGFPGAAGRVGPP | TCPC | 17.93 | 2+ | 680.3376 | 1,358.658 | 2.2 ppm | 95% |
| T2 | QPGFPGARGPAGGAP | TCPC | 15.99 | 2+ | 692.8358 | 1,383.653 | 2.8 ppm | 95% |
| T3 | QVGPAGLNAVQGSGAGAP | TCPC | 16.00 | 2+ | 783.9045 | 1,565.78 | 9.3 ppm | 93% |
| T4 | LAGAPGFRQP | TCPC | 17.28 | 2+ | 523.2755 | 1,044.535 | 1.3 ppm | 92% |
| T5 | FSGLDGKDAGAGGAP | TCPC | 24.64 | 2+ | 660.3176 | 1,318.615 | 4.1 ppm | 92% |
Abbreviations: ACC, Asini Corii Colla; ALC, average local confidence; CCC, Cervi Cornus Colla; GCM, Gelatinous Chinese medicine; MSD, mass spectrum detection; NanoLC, nano-flow liquid chromatography; TCPC, Testudinis Carapacis ET Plastri Colla; tR, retention time.