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. 2015 Apr 7;14(4):524–533. doi: 10.1111/acel.12305

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© 2015 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig 1 — Aging increases sensitivity to lipopolysaccharide (LPS)-induced changes to lipid metabolism. Lipopolysaccharide was injected intraperitoneally (2 mg kg⁻¹body weight) in young and aged rats. Rats were euthanized 12 h, 72 h, and 7 days after injection. (A) Measurement of triglycerides (TG) in serum following LPS injection in young and aged rats. (B) Measurement of free fatty acids (FFAs) in serum following LPS injection in young and aged rats. (C) Measurement of TG in the liver following LPS injection in young and aged rats. (D) Measurement of FFAs in the liver following LPS injection in young and aged rats. (E) Lipid accumulation in the livers was visualized by hematoxylin–eosin (H&E) staining. (F) Lipid accumulation in the livers was visualized by Oil red O staining. Oil red O positive areas were quantified. Data are expressed as the mean ± SEM. *P < 0.05 in comparison with the corresponding controls.