Fig 2.

Effects of aging on lipid metabolism-associated transcription factors changes induced by lipopolysaccharide (LPS) in liver. (A) The nuclear fraction of liver homogenates was used to detect transcription factors associated with lipid metabolism. Western blots were performed to detect nuclear protein levels of FXR, LXR, PPARα, PPARβ, PPARγ, SREBP1c, and SREBP2. TF-IIB was used as control. The blots of PPARα and SREBP1c were quantified by densitometry (n = 5). (B) qRT–PCR of PPARα target genes (Acox1, Cpt1, and Cyp4a1) in LPS-treated young and aged rat livers. mRNA levels were normalized to GAPDH level. (C) qRT–PCR of SREBP1c target genes (ACCa and FASN) in LPS-treated young and aged rat livers. mRNA levels were normalized to GAPDH level. Data are expressed as the mean ± SEM. *P < 0.05 vs. corresponding control group.