Fig 4.

Effects of aging on lipopolysaccharide (LPS)-induced liver inflammation. (A) Liver injury was quantified by measuring serum ALT/GPT levels. (B) Oxidative stress was measured using reactive species(RS)-specific fluorescent dye. (C) Activation of liver Kupffer cells was used as a marker of liver inflammation. Immunohistochemistry was used to evaluate recruitment of CD68 positive Kupffer cells. DAB-based brown-colored region indicates the CD68⁺ Kupffer cell. Percentage of Kupffer cells was calculated. Data are expressed as the mean ± SEM. *P < 0.05 vs. control group.