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. 2015 Apr 7;14(4):524–533. doi: 10.1111/acel.12305

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© 2015 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig 6 — IL-1β exerts lipogenic effects on hepatocytes through the regulation of PPARα and SREBP1c transcription factors. (A) Western blot for SREBP1c from HepG2 cells treated with IL-1β. Analysis of total lysates (upper panel) reveals upregulation of total SREBP1c (short exposure) as well as cleaved form of SREBP1c (long exposure). Actin was used as control. Analysis of nuclear fractions (lower panel) reveals increased translocation of SREBP1c after IL-1β treatment in HepG2 cells. TF-IIB was used as control. (B) Western blot for PPARα from HepG2 cells treated with IL-1β. TF-IIB was used as control. (C) Expression of SREBP1c target genes (ACCa, FASN) was analyzed by qPCR. Results were normalized to GAPDH level. (D) Expression of PPARα target genes (Cpt1, Acox1) was analyzed by qPCR. Results were normalized to GAPDH level.*P < 0.05 vs. control group.