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. 2015 Mar 10;14(4):569–581. doi: 10.1111/acel.12322

Fig 3.

Fig 3

Blocking TLR4 activation negatively affects expression of IL-8, IL-1β, and TNF-α genes. U937 cells were either co-treated with 0.2 μg mL-1 TLR4 antibody or transfected with a specific TLR4 siRNA for 24 h and then incubated with 6 μm 27-OH or 5 μm HNE for 6 h. Scramble siRNA (negative control) corresponds to a siRNA with nonspecific sequence. Expression of (A,B) IL-8, (C,D) IL-1β, and (E,F) TNF-α was analyzed by quantitative RT–PCR. The histograms represent the means values ± SD of three experiments. ***P < 0.001, **P < 0.01, and *P < 0.05 vs. control; §P < 0.05 vs. 27-OH or HNE; ##P < 0.01 and #P < 0.05 vs. 27-OH or HNE.