Fig 3.

BACE1 physically interacts with CHIP. (A) Schematic representation of CHIP and its deleted mutants used in the study. (B) Interaction between BACE1 and CHIP mutants. Two micrograms of Flag-BACE1 was co-transfected with 2 μg of myc-CHIP wild-type or its mutant. Co-immunoprecipitation assay was performed using anti-myc antibodies against CHIP, followed by Western blotting with anti-flag antibodies against the BACE1. BACE1 interacts with CHIP through its U-box domain. (C) Interaction between CHIP and BACE1 was independent of point mutations.