Fig 4.

Effects of senolytic agents on cardiac (A–C) and vasomotor (D–F) function. D+Q significantly improved left ventricular ejection fraction of 24-month-old mice (A). Improved systolic function did not occur due to increases in cardiac preload (B), but was instead a result of a reduction in end-systolic dimensions (C; Table S3). D+Q resulted in modest improvement in endothelium-dependent relaxation elicited by acetylcholine (D), but profoundly improved vascular smooth muscle cell relaxation in response to nitroprusside (E). Contractile responses to U46619 (F) were not significantly altered by D+Q. In panels D–E, relaxation is expressed as the percentage of the preconstricted baseline value. Thus, for panels D–F, lower values indicate improved vasomotor function. N = 8 male mice per group. *P < 0.05; A–C: t-tests; D–F: anova.