Fig 5.

Senolytic administration alleviates radiation-induced impairment in treadmill exercise endurance. (A–B) One leg of 4-month-old mice was radiated at 10 Gy. Three months later, hair on the irradiated leg had turned gray (A) and treadmill exercise capacity (B) was lower in irradiated (N = 13) than sham-irradiated mice (N = 14). **P < 0.002; t-test. (C) Five days after a single dose of D+Q, treadmill endurance was better than in vehicle-treated controls. D+Q had no effect in sham-irradiated controls. (N = 6-9 animals per group). Bars represent means ± SEM; *P < 0.05; **P < 0.001; anova; Tukey–Kramer test. (D) 7 months after a single dose of D+Q, treadmill endurance was again assayed. All groups ran on the treadmill on four occasions, each 1 week apart. Bars represent means ±SEM of the average performance of each group on each of the four occasions they ran. Endurance is shown as a function of the overall performance of all four groups on each occasion when mice ran (expressed as %: mean Joules per group/total Joules per all groups that day). * Different from the other groups; P < 0.05; anova; Duncan’s test.