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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Immunol Cell Biol. 2015 Feb 10;93(7):605–615. doi: 10.1038/icb.2015.9

Figure 2. Targeted deletion of the G0s2 gene.

Figure 2

(a) G0s2−/− mice were generated using Velocigene ES cells with deletion of the entire G0s2 gene. (b) Mice were genotyped by PCR using genomic DNA from tail biopsies and primers for the G0S2 hydrophobic domain and LacZ-gene insertion as indicated in (a). (c) Serum was collected from 8-10 week old mice for measurements of triglyceride, glucose, and free fatty acid (FFA) levels (n = 5). (d,e) Flow cytometric analysis of Gr1 and CD11b (granulocytes, monocytes), CD19 (B cells), and CD3 (T cells) content in the peripheral blood of 10-12 week old mice. *, P < 0.05 (two-tailed Student’s t-test).