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. 2015 Aug 11;9:315. doi: 10.3389/fncel.2015.00315

FIGURE 2.

FIGURE 2

Cav2.1 channels are organized in discrete groups in the presynaptic active zone of boutons in inhibitory GABAergic and excitatory glutamatergic cells in the stratum radiatum of the hippocampal CA1 area. (A) Electron micrograph of a replica double-labeled for vesicular GABA transporter (VGAT; 10 nm gold particles) and VGLUT-1 (15 nm) showing no overlap between the two subpopulations. (B) Quantification of gold particles further demonstrated that 15% of the examined axon terminals (n = 328) were VGAT+ (bVGAT+) and 85% were immunoreactive for VGLUT-1 (bVGLUT-1+). (C–H) Replica images showing aggregation of immunogold particles labeling Cav2.1 (10 nm) in small (C,E,G) and large (D,F,H) active zones of VGAT+ (15 nm; C,D), VGAT- (E,F) and VGLUT-1+ (15 nm; G,H) boutons (b). Note that VGAT- and VGLUT-1+ terminals make asmmetrical synapses with dendritic spines (s in E,F,G) that can be recognized by the high density of IMPs on the E-face of the plasma membrane. Scale bars, 0.2 μm.