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. 2015 Aug 11;6:615. doi: 10.3389/fpls.2015.00615

Figure 2.

Figure 2

Analysis of the transactivation activity and subcellular localization of TaWRKY44. (A) Transactivation activity of the TaWRKY44 protein in yeast. Schematic diagrams of fused vectors illustrating the different portions of TaWRKY44 that were fused to the yeast vector pGBKT7. Yeast strain AH109 was used in the transactivation activity analysis of TaWRKY44. The transformants were incubated on the SD/-Trp or SD/-His medium and subjected to X-α-gal assay. Three biological experiments produced similar results. (B) Subcellular localization of the TaWRKY44 protein in onion epidermal cells. The fusion protein TaWRKY44-GFP (pBI121- TaWRKY44-GFP) and GFP (pBI121-GFP) were transiently expressed in the onion epidermis using the bombardment method. Pictures were taken in bright and fluorescence fields after DAPI staining with fluorescence microscopy 24 h after bombardment. Three biological experiments were carried out, which produced similar results.