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. 2015 Aug 11;6:615. doi: 10.3389/fpls.2015.00615

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Copyright © 2015 Wang, Zeng, Li, Rong, Sun, Sun, Li, Wang, Feng, Chai, Chen, Chang, Li, Yang and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the W-box binding activity of TaWRKY44 using a yeast one-hybrid system. (A) Schematic diagrams of the effector plasmids (pGAD-TaWRKY44 and pGADT7) and reporter plasmids (pHIS2-(m) W-box and pHIS2) used for the yeast one-hybrid assay. (B) Schematic diagrams of vector transformation combinations and the growth of yeast cells on SD/-His/-Leu/-Trp supplemented with (30 and 60 mM) or without 3-AT. The mW1-5 and W-box sequence indicated the core sequence of each vector. Three biological experiments produced similar results.