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. 2015 Aug 11;6:615. doi: 10.3389/fpls.2015.00615

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Copyright © 2015 Wang, Zeng, Li, Rong, Sun, Sun, Li, Wang, Feng, Chai, Chen, Chang, Li, Yang and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression levels of ROS-related and stress-responsive genes in the WT, VC and the transgenic lines (OE-35) under normal and osmotic conditions analyzed qRT-PCR. Two-week-old seedlings cultured on MS medium were transplanted to MS with mannitol (300 mM) and NaCl (200 mM) for 1 week. The tobacco leaves were then sampled to extract the total RNA to generate cDNA. Fourteen gene expression levels were detected; the NtActin gene was used as the internal control for normalization. Data are means ±SD calculated from three replicates. ^*(P < 0.05) and ^**(P < 0.01) indicate that the value in the transgenic line is significantly different from that of the WT. Three biological experiments produced similar results.