Table 7.
Differentially expressed proteins identified by the quantitative proteomic approach: proteins involved in stress response.
| Biological process | Protein name | Gene | Antibiotics | Regulation | Frequency of difference | References | Protein description |
|---|---|---|---|---|---|---|---|
| General stress-related proteins | Cold shock protein | cspA | Met | Up | 1 | Cordwell et al., 2002 | Cold shock tolerance |
| Dps family protein | dps | Van | Down | 1 | Ramos et al., 2015 | Protection of DNA from damage | |
| Two-component regulator protein | vanR | Van | Up | 1 | Ramos et al., 2015 | The VanS/VanR two-component system in response to extracellular glycopeptide antibiotic | |
| Lactoylglutathione lyase | gloA | Van | Up | 1 | Wang et al., 2010 | Methylglyoxal degradation | |
| Cell stress stimulon response regulator | vraR | Van | Up | 2 | Scherl et al., 2006; Drummelsmith et al., 2007 | The two-component regulatory system VraS/VraR involved in the control of the cell wall peptidoglycan biosynthesis | |
| HTH-type transcriptional regulator | sarS | Van | Down | 1 | Drummelsmith et al., 2007 | Transcriptional regulator that controls expression of some virulence factors in a cell density-dependent manner | |
| Accessory gene regulator A | agrA | Van | Down | 2 | Scherl et al., 2006; Drummelsmith et al., 2007 | The regulation of virulence proteins | |
| Signal transduction protein TRAP | traP | Van | Up | 1 | Scherl et al., 2006 | A major regulator of staphylococcal pathogenesis | |
| Thioredoxin reductase | trxB | Van | Down | 1 | Drummelsmith et al., 2007 | Thioredoxin pathway | |
| Competence protein | cglA | Lin | Down | 1 | Feng et al., 2011 | Competence regulation | |
| Competence protein | cglB | Lin | Down | 1 | Feng et al., 2011 | Competence regulation | |
| Phosphate transporter | phoU | Lin | Down | 1 | Feng et al., 2011 | Phosphate starvation | |
| Conserved membrane protein; phage-shock protein A homolog (three-component regulatory system) | liaIH | Dap | Up | 1 | Wecke et al., 2009 | Regulation of membrane permeability | |
| Undecaprenyl pyrophosphate phosphatase | bcrC | Dap | Up | 1 | Wecke et al., 2009 | Bacitracin resistance | |
| Superoxide dismutase | sodM | Met | Up | 2 | Cordwell et al., 2002; Enany et al., 2014 | Resistance to oxidative stress | |
| Van | Down | 1 | Drummelsmith et al., 2007 | ||||
| Competence damage-inducible protein A | cinA | Van | Up | 1 | Pieper et al., 2006 | Competence regulation | |
| Lin | Down | 1 | Feng et al., 2011 | ||||
| Two-component sensor histidine kinase | vraS | Van | Up | 1 | Scherl et al., 2006 | The two-component regulatory system VraS/VraR involved in the control of the cell wall peptidoglycan biosynthesis | |
| Dap | Up | 1 | Fischer et al., 2011 | ||||
| Staphylococcus accessory regulator A | sarA | Van | Up | 1 | Drummelsmith et al., 2007 | Regulation of the virulence factors | |
| Dap | Up | 1 | Fischer et al., 2011 | ||||
| GTP pyrophosphokinase | relA | Van | Down | 1 | Drummelsmith et al., 2007 | Stringent response | |
| Dap | Up | 2 | Wecke et al., 2009; Fischer et al., 2011 | ||||
| Choline dehydrogenase | betA | Van | Up | 1 | Scherl et al., 2006 | Glycine betaine biosynthesis | |
| Dap | Down | 1 | Fischer et al., 2011 | ||||
| GTP-sensing transcriptional pleiotropic repressor | codY | Lin | Up | 1 | Feng et al., 2011 | Transcription regulation in response to the GTP level | |
| Dap | Up | 1 | Fischer et al., 2011 | ||||
| Alkaline shock protein 23 | asp23 | Met | Up | 2 | Cordwell et al., 2002; Enany et al., 2014 | Alkaline pH tolerance | |
| Van | Down | 1 | Hessling et al., 2013 | ||||
| Dap | Up | 1 | Fischer et al., 2011 | ||||
| Catalase | katA | Met | Up | 1 | Cordwell et al., 2002 | Resistance to oxidative stress | |
| Van | Up | 1 | Scherl et al., 2006 | ||||
| Dap | Up | 1 | Fischer et al., 2011 | ||||
| Superoxide dismutase | sodA | Met | Up | 2 | Cordwell et al., 2002; Enany et al., 2014 | Resistance to oxidative stress | |
| Van | Up | 1 | Wang et al., 2010 | ||||
| Lin | Up | 1 | Feng et al., 2011 | ||||
| Cold shock protein | cspB | Met | Up | 1 | Cordwell et al., 2002 | Cold shock tolerance | |
| Van | Down | 1 | Drummelsmith et al., 2007 | ||||
| Dap | Up | 1 | Fischer et al., 2011 | ||||
| Cold shock protein | cspC | Met | Up | 1 | Cordwell et al., 2002 | Cold shock tolerance | |
| Van | Down | 1 | Drummelsmith et al., 2007 | ||||
| Dap | Up | 1 | Fischer et al., 2011 | ||||
| Alkyl hydroperoxide reductase subunit C | ahpC | Met | Up | 1 | Enany et al., 2014 | Resistance to oxidative stress | |
| Van | Up | 2 | Scherl et al., 2006; Chen et al., 2013 | ||||
| Dap | Up | 1 | Fischer et al., 2011 | ||||
| Virulence-related proteins | Secreted virulence factor | esxA | Van | Down | 1 | Drummelsmith et al., 2007 | Pathogenesis |
| Extracellular ECM and plasma binding protein | ssp | Van | Up | 1 | Scherl et al., 2006 | Pathogenesis | |
| Cell surface-associated protein | sdrE | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| Clumping factor A | clfA | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| Secretory extracellular matrix and plasma binding protein | empbp | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| Enterotoxin type I | sei | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| Cysteine protease precursor | sspB1 | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| Leukotoxin | lukD | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| Leukotoxin | lukE | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| Phospholipase C | hlb | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| HysA | hysA | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| γ-hemolysin, component C | hlgC | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| Lipase | geh | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| Accessory protein Z | sarZ | Van | Down | 1 | Hessling et al., 2013 | Pathogenesis | |
| α-hemolysin | SAV1163 | Lin | Down | 1 | Bernardo et al., 2004 | Pathogenesis | |
| Respiratory response protein | srrA | Met | Up | 1 | Cordwell et al., 2002 | Pathogenesis | |
| Van | Down | 1 | Scherl et al., 2006 | ||||
| Fibrinogen-binding protein | efb | Met | Up | 1 | Enany et al., 2014 | Pathogenesis | |
| Van | Down | 1 | Hessling et al., 2013 | ||||
| Immunoglobulin G binding protein A | spa | Van | Down | 2 | Pieper et al., 2006; Drummelsmith et al., 2007 | Pathogenesis | |
| Lin | Down | 1 | Bernardo et al., 2004 | ||||
| Bifunctional autolysin | atl | Van | Up | 1 | Drummelsmith et al., 2007 | Pathogenesis; Cell wall biogenesis/degradation | |
| Lin | Down | 1 | Bernardo et al., 2004 | ||||
| Dap | Up | 1 | Fischer et al., 2011 | ||||
| Immunodominant antigen A | isaA | Met | Up | 1 | Cordwell et al., 2002 | Pathogenesis; Cell wall biogenesis/degradation | |
| Van | Up | 3 | Scherl et al., 2006; Drummelsmith et al., 2007; Chen et al., 2013 | ||||
| Lin | Down | 1 | Bernardo et al., 2004 | ||||
| Dap | Up | 1 | Fischer et al., 2011 |