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. Author manuscript; available in PMC: 2016 Jan 16.
Published in final edited form as: Nature. 2015 Jul 8;523(7560):361–365. doi: 10.1038/nature14613

Figure 1. Evolution of the pheromone response in budding yeast.

Figure 1

a, During mating, cells sense a peptide pheromone (brown molecule) and transmit this signal to the transcription factor Ste12. b, K. lactis a cells were induced with 10μg/mL synthetic α-factor pheromone, and gene expression was measured by quantitative RT-qPCR over time. Shown are mean values of three independent genetic isolates +/− s.d. c, Conserved pheromone responsive genes were scored for the presence of Ste12 cis-regulatory motifs in their upstream regulatory regions. The track indicates the −log10(P) for the enrichment of the Ste12 motif in each set of genes in each species, by hypergeometric distribution. The genes were divided into a-specific genes and general pheromone-activated genes. The general pheromone-activated genes were used to independently generate the Ste12 cis-regulatory motif in each of four clades using MEME.