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. 2015 Jul 24;4:e08298. doi: 10.7554/eLife.08298

Figure 2. Starvation-dependent neuropeptide signaling modulates sensitivity of the DM1 and DM5 glomeruli.

(A) Representative two photon images of projection neuron (PN) dendritic calcium responses to 80% saturated vapor (SV) of apple cider vinegar in starved DTKR knockdown flies. Grey-scale images show the glomerular map on three optical planes whereas the pseudocolored images show the change of fluorescence. (B) Peak ΔF/F in glomeruli that are activated by 80% SV cider vinegar in fed and starved flies. (C, D) Responses in the DM1 and DM5 glomeruli at varying concentrations in fed and starved control flies (C) and in flies that express DTKR-RNAi or sNPFR-RNAi in ORNs labeled by Orco-Gal4 (D). Calcium signals for PN responses were imaged using GH146-LexA, LexAop-GCaMP flies in addition to the indicated transgenes. n = 5–7 for each. Error bars show s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001; Student's t-test comparing between starved and fed responses.

DOI: http://dx.doi.org/10.7554/eLife.08298.006

Figure 2—source data 1. Glomerular responses to vinegar in fed and starved flies.
DOI: http://dx.doi.org/10.7554/eLife.08298.007
elife08298s001.docx (18.8KB, docx)
DOI: 10.7554/eLife.08298.007

Figure 2.

Figure 2—figure supplement 1. PN responses to vinegar in flies with DTKR knockdown.

Figure 2—figure supplement 1.

Two-photon imaging of PN dendritic calcium responses. (A) Peak ΔF/F in glomeruli that are activated by 80% SV cider vinegar in control and DTKR knockdown flies. (B) Peak ΔF/F in the DM1 glomerulus in response to 0.2% SV cider vinegar in control and DTKR knockdown flies. (C) Representative images of calcium responses to 0.4% SV of ethyl butyrate (1:100 dilution in mineral oil). Grey-scale images show the glomerular map on the DM5 plane whereas the pseudocolored images show the change of GCaMP fluorescence. (D) Peak ΔF/F in the DM5 glomerulus in response to ethyl butyrate in control and DTKR knockdown flies. All flies have GH146-LexA, LexAop-GCaMP, Orco-Gal4, and DTKRi flies have UAS-DTKR-RNAi in addition. For each condition, n = 4–9. Error bars show s.e.m. *p < 0.05, **p < 0.01; Student's t-test comparing the response between knockdown and control groups (A) and comparing between starved and fed responses (B, D).
DOI: http://dx.doi.org/10.7554/eLife.08298.008