Figure 3. Tachykinin released by antennal lobe local interneurons (LNs) is necessary for starvation-dependent suppression of DM5 glomerular activity.
(A) Representative traces showing ΔF/F in the DM5 glomerulus in flies that have UAS-DTK-RNAi in ORNs (Orco-Gal4) or in LNs (GH298-Gal4). Bar graph depicts peak ΔF/F for each indicated genotype. Calcium signals for PN responses were imaged using GH146-LexA, LexAop-GCaMP flies in addition to the indicated transgenes. n = 5 for each condition. (B) The appetitive index of DTK knockdown flies in response to 5% cider vinegar. n = 87–101 for each condition. For imaging experiments, Error bars show s.e.m. *p < 0.05, Student's t-test (A) and z-test for proportions (B) comparing GH298-Gal4, UAS-DTK-RNAi group to control groups or Orco-Gal4, UAS-DTK-RNAi group.