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. 2015 Jun 19;27(7):1985–1998. doi: 10.1105/tpc.114.135780

Figure 4.

Figure 4.

The Severe RanGAP Knockdown Mutant TWEED Is Defective in Cell Division and Meristem Cell Identity but Shows No Aberrations in Nucleocytoplasmic Transport.

(A) Expression of PromCyclinB1;1:GUS in 7-d-old Col-0, SILK, and TWEED seedlings. While staining was variable within the TWEED line, TWEED seedlings showed a substantial reduction, and SILK seedlings showed only a slight reduction in GUS staining when compared with Col-0. Seedlings were stained for 4 h. Bar = 100 μm.

(B) WOX5:GFP localization in 7-d-old Col-0, SILK, and TWEED roots stained with FM4-64. TWEED mutant seedlings display weaker WOX5:GFP signal and mispositioned WOX5:GFP expressing cells when compared with Col-0 and SILK mutant seedlings. Bar = 20 μm.

(C) N7-GFP localization in Col-0, SILK, TWEED and inducible Ran1T27N seedlings. N7-GFP is localized to the nucleus in Col-0, SILK, TWEED, and uninduced Ran1T27N seedlings. By contrast, the fluorescent fusion protein accumulated in the cytoplasm in estradiol-induced Ran1T27N seedlings. Bar = 20 μm.