Figure 6.

The GAP Activity of RanGAP1 Is Necessary for Its Role in Silique Elongation and Sporophyte Development.

(A) Silique development depends on the GAP activity of RanGAP1. The silique length for RanGAP1 and its mutant variants in the SILK background. Box represents the 1st and 3rd quartiles of the data set, middle line represents the mean, and whiskers represent the 95th and 5th percentiles. The mean of the data set is represented by a gray X. Boxes in light gray are statistically different from those in dark gray, according to ANOVA followed by the Tukey’s honestly significant difference post-hoc test (see Supplemental Table 1 for test results).

(B) Images of rescued T5 rg1-1/rg1-1 rg2-3/rg2-3 (double null mutant) plants containing various RanGAP1 variant constructs and mutant and wild-type controls grown in parallel. Each line represents an independent transformant. Two plants per line are shown, except for RanGAP1^AAP+N219A. The second double null mutant individual identified containing the RanGAP1^AAP+N219A variant was sterile and is pictured in Supplemental Figure 10. DN, double null mutant. Bars = 2 cm.