Figure 1.

Generation and Verification of mtLPD Overexpressors.

(A) Schematic overview of the mtLPD overexpression construct.

(B) PCR verification of the transformed construct into the genome of transgenic lines (B1) and the corresponding loading control (B2).

(C) RT-PCR verification of the full-length mtLPD transcript (C1) using signals of the constitutively expressed 40S ribosomal protein S16 gene as calibration control (C2).

(D) Immunoblot of leaves, roots, and isolated mitochondria using a specific antibody against mtLPD and mitochondrial malate dehydrogenase (mMDH) as loading control.

(E) Enzymatic activity of mtLPD in overexpressor and wild-type mitochondria. Values are means ± sd from four technical replicates. Asterisks indicate values that were significantly different from the wild-type control based on Student’s t test (*P < 0.05).