Figure 5.

Respiratory Gas Exchange and Mitochondrial Respiration of mtLPD Lines.

(A) Fully expanded leaves from plants at growth stage 5.1 (Boyes et al., 2001) were used for determination of the respiratory CO₂ release during illumination and after dark adaptation (2 to 4 and 12 to 14 h, respectively; n > 4) by gas-exchange measurements.

(B) Malate and glycine respiration of intact mitochondria from mtLPD overexpressors and the wild type (n = 4). Respiratory coupling of mitochondria was verified by determination of respiratory control values (RC) from the transition from state 3 to state 4. RC values were in the range of previous reports (Keech et al., 2005) confirming that isolated organelles were intact and of good quality. Asterisks indicate values significantly different from the wild-type control based on Student’s t test (*P < 0.05).