Table 3. Rubisco Activity in mtLPD Overexpressors Compared to the Wild Type.

Parameter	Col-0	PsL-L1	PsL-L2	PsL-L3
Total protein	9.88 ± 0.47	10.64 ± 1.23	10.74 ± 0.60	10.01 ± 1.12
Total Rubisco	20.49 ± 0.64	20.79 ± 0.82	21.18 ± 0.77	20.63 ± 0.56
Rubisco/protein	47.40 ± 1.53	47.82 ± 1.88	48.84 ± 1.61	47.52 ± 1.24
Rubisco (initial)	143.63 ± 12.36	137.92 ± 19.87	150.11 ± 12.81	135.43 ± 13.13
Rubisco (total)	202.91 ± 23.39	175.37 ± 17.31	186.14 ± 23.61	176.96 ± 8.53
Rubisco activation	70.09 ± 3.26	78.40 ± 4.24	81.14 ± 6.61	76.61 ± 7.64

Leaves from plants at growth stage 5.1 (Boyes et al., 2001) were harvested in the middle of the light period (5 h illumination) as used for all other physiological experiments (10/14-h day/night, 390 ppm CO₂, 20/18°C day/night, 120 µmol m⁻² s⁻¹ light intensity, and ∼70% relative humidity). Values presented are means ± sd of measurements from at least five biological and two technical replicates per genotype. Numbers in bold indicate values statistically significant from the wild type based on Student’s t test (*P < 0.05). Units are as follows: total protein (µg mg⁻¹ fresh weight), total Rubisco (µg cm⁻² leaf area), Rubisco/protein and Rubisco activation (%), and Rubisco activity (nmol min⁻¹ cm⁻² leaf area).