Table 4. Summary of Candidate Gene Association Results for AGT and TDC2 Based on Resequencing.
| Gene | Trait | Marker | Chromosome | Position (bp)a | P (leaf1)b | P (leaf2)c | Region | Codon change | Amino acid replacement |
|---|---|---|---|---|---|---|---|---|---|
| AGT | β-Alanine | InDel_15353417 | 1 | 15353417 | 5.19 × 10−4 (297) | 3.81 × 10−8 (298) | 5′ UTR | ||
| chr1.S_15353577 | 1 | 15353577 | 0.02 (339) | 1.11 × 10−6 (339) | Exon | C/T | Ala-Val | ||
| chr1.S_15353647 | 1 | 15353647 | 4.23 × 10−3 (339) | 10.05 × 10−7 (339) | Exon | T/C | |||
| chr1.S_15353690 | 1 | 15353690 | 4.33 × 10−4 (339) | 6.62 × 10−7 (339) | Exon | T/C | Tyr-His | ||
| chr1.S_15353704 | 1 | 15353704 | 4.33 × 10−4 (339) | 6.62 × 10−7 (339) | Exon | A/G | |||
| chr1.S_15355785 | 1 | 15355785 | 6.12 × 10−4 (339) | 0.01 (339) | Exon | A/G | Asp-Gly | ||
| TDC2 | Tyramine | InDel_17855186 | 1 | 17855186 | 2.6 × 10−8 (282) | 9.2 × 10−9 (272) | 5′ UTR | ||
| Dopamine | InDel_17855186 | 1 | 17855186 | 0.02 (205) | 0.002 (234) | 5′ UTR |
a
The physical position of each polymorphic site in the maize genome.
b
P value for the association between each marker and the corresponding metabolite level in leaves at seedling stage; association analysis was conducted using the MLM accounting for population structure (Q) and kinship (K); number of lines used in the association analysis is indicated in the parentheses.
c
P value for the association between each marker and the corresponding metabolite level in leaves at reproductive stage; association analysis was conducted using the MLM accounting for population structure (Q) and kinship (K); number of lines used in the association analysis is indicated in the parenthesis.