Table 2. Genotyping Primers and Markers.
| Size of Products (bp) |
|||||||
|---|---|---|---|---|---|---|---|
| Marker | Gene | Enzyme | abp1-5 | Col-0 | Ws-2 | Ler-0 | Oligonucleotidesa |
| ABP1 | At4g02980 | RsaI | 107+43 | 150 | 150 | 150 | CATTGGTATCCGCTCGGCTCTTATCTGTG |
| GTACCACTGCCCTTTAGGACAAC | |||||||
| PHYB | At2g18790 | PstI | 155 | 130+25 | 130+25 | 130+25 | AAGGTATGCTTGTGAGTTTTTGCTG |
| GTGTAACAATTCCAGCAGGCGAGTC | |||||||
| At1g23880 | At1g23880 | AccI | 190 | 169+21 | 169+21 | 169+21 | GTATTCAGTGGAAACGGTGGTA |
| TGAAATCCACCAAGTCTGACGCAAAAT | |||||||
| At2g39240 | At2g39240 | XhoI | 150 | 130+20 | 130+20 | 130+20 | GAGAGACTCCTGAAGATGCCCTCGA |
| TGCATACAAAATCAAGTCCAATAAT | |||||||
| At3g09530 | At3g09530 | HinfI | 199 | 178+21 | 199 | 178+21 | CCAACAATCTCCAACACGTTGACT |
| GCTTCTTCGGGCGACATCTCCAC | |||||||
| At5g59900 | At5g59900 | AccI | 200 | 176+24 | 176+24 | 176+24 | GTGAGCTAGTCTCGAGAAGATTGTAGA |
| CAACAGTGATGAAGCAGGCCAGAAGAGATTA | |||||||
| nga162 | At3g13960 | – | 85 | 107 | 85 | 89 | CATGCAATTTGCATCTGAGG |
| CTCTGTCACTCTTTTCCTCTGG | |||||||
| nga112 | At3g62650 | – | 189 | 197 | 189 | 189 | CGTGTATGCAGCTGCATAGACAGTGG |
| GGCGTTATCTCCATCACTCCCTATAGC | |||||||
| GLL310 | At3g51380 | – | 204 | 246 | 204 | 204 | AGAAGAGACAGTGACAGAATCGGGTAATAAG |
| GTCTATCTCCCCCACTTGTTCATC | |||||||
| GLL320 | At3g50550 | ApoI | 191 | 191 | 191 | 114+77 | GAATGGCTAGCCCCAAAGAC |
| TATTGCGTAAAGAAGCGAAAAC | |||||||
| LCS332 | At3g17850 | DpnII | 148 | 118+30 | 148 | 148 | CTGATGGCGGCAAAGTAGGGCTGAG |
| CGAAGATGGGTGCTGATGGTGGTGAC | |||||||
a
Underlined nucleotide is the introduced mutation for the derived cleaved amplified polymorphic sequence marker (Neff et al., 2002).