SWP73B Is Involved in Leaf Development.
(A) Leaves of 7-week-old wild-type and swp73a plants show normal development, while the swp73b mutant exhibits severe defect of leaf development, including a great reduction of palisade parenchyma layer. Bars = 1 cm (left panel) and 1 mm (right panel).
(B) Comparison of expression levels of genes involved in leaf development in 21-d-old wild-type, swp73a-1, and swp73b-1 plants. Asterisks indicate significant differences from wild-type plants (error bars refer to sd, P < 0.05, Student’s t test, three biological and three technical replicates were used).
(C)
ChIP-qPCR analysis of SWP73B cross-linked promoter regions of AS1, AS2, KAN1, KAN3, YAB2, YAB3, and YAB5 in wild-type and SWP73B-YFPHA plants using the TA3 retrotransposon (AT1G37113) as reference. Asterisks indicate significant differences from wild-type plants (error bars refer to sd, P < 0.05, Student’s t test, three biological and three technical replicates were used).