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. 2015 Jun 23;27(7):1889–1906. doi: 10.1105/tpc.15.00233

Figure 6.

Figure 6.

Effects of Concomitant Inactivation of SWP73A and SWP73B on Embryo Development.

(A) Comparison of open siliques of wild-type and swp73a swp73b-1/+ plants. The occurrence of white aborted seeds demonstrates embryo lethality; arrested ovules indicate female gametophyte lethality. Bar = 0.5 mm.

(B) Nomarski images of green wild-type-like and white swp73a swp73b seeds at the same stage of development and arrested ovules. The inspection indicated regular heart-stage embryos in wild-type-looking green seeds. White translucent seeds contained an aberrant globular-stage embryo. Bar = 10 µm.

(C) Three-weeks-old LD-grown swp73a-1+swp73b-1 plants exhibit ectopically positioned meristems and aberrant shape cotyledons and leaves. Bar = 1 mm.

(D) Comparison of 4-week-old LD-grown wild-type, swp73b-1, and swp73a/+swp73b mutants indicates strong growth retardation of swp73a/+swp73b plants. Bar = 1 cm.

(E) Examination of pollen viability in the anthers of swp73a-1, swp73b-1, swp73a swp73b/+, and swp73a/+swp73b/+ lines by Alexander staining. Red staining, viable pollen grains; green staining, dead pollen grains. Bar = 10 µm.