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Detection of total anti-HER2/neu immunoglobulin in the form of gemcitabine-(C4-amide)-[anti-HER2/neu] bound to the exterior surface membrane of chemotherapeutic-resistant mammary adenocarcinoma (SKBr-3). Covalent gemcitabine-(C4-amide)-[anti-HER2/neu] immunochemotherapeutic was incubated with monolayer populations of mammary adenocarcinoma (SKBr-3) over a 4-hour period and total immunoglobulin bound to the exterior surface membrane was measured by cell-ELISA.
