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. Author manuscript; available in PMC: 2016 Jul 13.
Published in final edited form as: Cancer Cell. 2015 Jul 13;28(1):97–113. doi: 10.1016/j.ccell.2015.06.004

Figure 1. DNA-PKcs binds AR and is recruited to sites of AR action.

Figure 1

(A) C4-2 cells were treated with ADT (CSS) for 24 hrs and immunoblot analysis for phospho-S2056 DNA-PKcs, total DNA-PKcs, and Ku70, performed. (B,C) C4-2 cells in hormone proficient media were (B) harvested for ChIP-qPCR analysis and percent (input) occupancy of AR (left) or DNA-PKcs (right) reported or (C) treated with 10nM DHT and harvested for ChIP-qPCR analysis with percent (input) occupancy of AR, DNA-PKcs, p300, or RNPII set relative to control at each time point. (D) C4-2 cells were treated with 10nM DHT and relative transcript expression analyzed as normalized to GAPDH mRNA at each timepoint. (E,F) C4-2 cells were treated with 10nM DHT for 6 hrs and co-immunoprecipitation performed in the absence (E) or presence (F) of 50μg/mL ethidium bromide. Data are reported as mean +/− SD. *p<0.05 **p<0.01. See also Fig S1.

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