Figure 2. DNA-PKcs selectively impacts gene expression in CRPC.
(A) RNA harvested from C4-2 cells depleted of DNA-PKcs or treated with 1μM NU7441 (DNA-PKcsi) for 24 hrs was analyzed by microarray analysis (left). Immunoblot of phospho-S2056 DNA-PKcs, total DNA-PKcs, and Ku70 after knockdown or NU7441 treatment (right). (B) Genes identified as upregulated (left) or downregulated (right) by ≥1.5 fold compared to untreated. (C,D) GSEA motif (left) or gene ontology (right) analyses of all genes altered at least 1.5-fold after DNA-PKcs knockdown.