Figure 6. DNA-PKcs inhibitors delay formation of metastases in vivo.

(A) Mice were injected with luciferin 31 days post tail vein injection of PC3-ML cells and imaged using the IVIS imaging system with total luciferase signal reported (left) and representative images shown (right). Indicated mice were selected for crossover studies. (B,C) Mice were injected with luciferin and imaged for 2 weeks after initiation of crossover studies with total luciferase signal reported (left), representative images shown (middle), and average doubling times pre and post crossover calculated. (D) CASP-NPK-YFP tumors were measured twice weekly for 30 days after initiation of treatment (end point for survival was the predefined tumor volume of 1.5cm³) with volumes calculated using the formula volume=(width)²xlength/2. (E) At time of sacrifice, metastases were documented ex vivo in the lungs and livers by visualizing fluorescence with the total number of metastatic nodules for the lungs and livers assessed. (F) CASPNPK-YFP tumors were harvested for qPCR analysis with the indicated transcripts set relative to Gapdh mRNA. Data are reported as mean +/− SD. **p<0.01. See also Fig S5.