Table 3.
Gene primers
| Gene symbol | Gene name | Forward and reverse |
|---|---|---|
| GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | GGGAAACCCATCACCATCTT |
| CCAGTAGACTCCACGACATACT | ||
| BDNF | Brain-derived neurotrophic factor | GAGACAAGAACACAGGAGGAAA |
| CCCAAGAGGTAAAGTGTAGAAGG | ||
| FTO | Fat mass and obesity-associated protein | CTGTGGAAGAAGATGGAGAGTG |
| CAGGACGGCAGACAGAATTT | ||
| GAL | Galanin | CCATTGACAACCACAGATCATTTA |
| CAACACTTCCTAGTCTCCCTTC | ||
| GALR1 | Galanin receptor 1 | GTTCCCATAGGTGTACAGAGTTC |
| GGTGTCTTAGTCCACAGGATTAC | ||
| GALR2 | Galanin receptor 2 | GGACCAAAGGGCATCTAACA |
| CCTACAATCCTCGGTCTTTAGC | ||
| HAT1 | Histone acetyltransferase 1 | TGTTTCTCCCGGGAAAGATTAC |
| CCCGTCTAGCATGTTGCTTAT | ||
| HDAC2 | Histone deacetylase 2 | CTGTCAAAGGTCACGCTAAATG |
| GTCCAACATCGAGCAACATTC | ||
| HDAC4 | Histone deacetylase 4 | AGCTGCAGGAGTTTGTTCTC |
| CTGTGCTGTGTCTTCCCATAC | ||
| INSR | Insulin receptor | CCCTGTGACCCATGAAATCTT |
| CGCCGATAGCTCACTTCATATAG | ||
| OBRB | Leptin receptor, long form | GGTTGGATGGACTAGGGTATTG |
| CAGAATTCAGGCCCTCTCATAG | ||
| ORXR | Orexin receptor | CTCCTCATCGTGACACTGAAAG |
| GAGGAAGAGAAACTCCCACAAG | ||
| RHEB1 | Ras homolog enriched in brain 1 | GAGCCCACCACCTCAATAAT |
| GGGAAAGTGCAGATACCGATTA | ||
| SOCS3 | Suppressor of cytokine signaling 3 | ACCTTTCTTATCCGCGACAG |
| CACTGGATGCGTAGGTTCTT | ||
| SYN1 | Synapsin 1 | GGACGGAAGGGATCACATTATT |
| ACCACAAGTTCCACGATGAG |
Sequences for all primers used in qRT-PCR reactions.