Fig. 1.

The 3D model of the covalently cross-linked TNF07 mutein. a Top view of the S95C/G148C double mutant. The three monomers are shown in cartoon representation and the monomers are colored in blue, purple and green. The surface representation is also shown in light grey. Red rectangles highlight the three Cys-Cys double bonds between each monomer. Residues 95 and 148 are shown in ball and stick representation. b Side view of the S95C/G148C double mutant. Red rectangle highlights one of the Cys-Cys double bonds and the insert shows the close up view. The model was generated by Modeller (v.9.12) [24] using the structure of mouse TNF (PDB ID: 2TNF) as the template. The pictures were created by the program PyMOL [25]