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Stimulation of cAMP production by bTSH or pooled Graves’ IgG in Mc2 cell line. Stimulation of cAMP by pooled Graves’ IgG (5 g/L) was negligible when compared to that by bTSH in Mc2 cell line expressing mutant TSH receptors, in which a.a. residue 90–165 of human TSH receptor was replaced by those of rat LH/CG receptors. Graves’ IgG were prepared from pooled sera of 30 untreated Graves’ patients.
