Skip to main content
. 2015 Aug 11;5:12931. doi: 10.1038/srep12931

Figure 4. Liver fibrosis in wt and ApoE−/− mice with or without WD or MCD diet.

Figure 4

(A) Gene expression of profibrotic markers TGFβ and collagen 1 (Col1A), as a marker of hepatic collagen deposition and liver fibrosis. Levels of profibrotic markers were elevated in ApoE−/− fed with Western Diet (WD) compared to wt mice fed with either WD or methionine and choline deficient (MCD) diet. ApoE−/− mice fed with MCD diet showed marked elevation in levels of profibrotic markers on mRNA level. (B) Hepatic hydroxyproline content as a marker of fibrosis was significantly elevated in ApoE−/− mice fed with WD compared to wt mice either fed with WD or MCD diet. (C) Representative sections and (D) quantification (% of positive stained area) of Sirius red histological staining. ApoE−/− mice fed with WD showed significantly more Sirus red positive staining than wt mice fed with WD. In mice fed with MCD diet Sirius red positive staining was comparable to ApoE−/− mice fed with WD. Graphs are expressed as means ± standard deviation. The scale bar is 50 μm respectively 20 μm for the magnifications. p < 0.05 was considered significant.